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Promega pgem®-t easy vectors
Pgem® T Easy Vectors, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgem-t+vectors/pgem+t+easy/us12358989-636-14-17
Average 90 stars, based on 1 article reviews
pgem®-t easy vectors - by Bioz Stars, 2026-09
90/100 stars

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Purification:

Article Title: Stemness Activity Underlying Whole Brain Regeneration in a Basal Chordate.
Article Snippet: Genes were amplified from P. mytiligera cDNAs using gene-specific primers and cloned into pGEM-t vectors using the manufacturer’s protocol (Promega; CAT #A1360).

Article Title: Engineering redirected NF-κB/OIP5 expression programs to enhance tumor responses to chemotherapy in bladder cancer.
Article Snippet: The PCR products were then subcloned using the pGEM-T vectors (Promega, USA) and transformed into DH5a E. coli cells.

Article Title: Two neuropeptide signaling pathways regulate post-mating refractoriness and reproductive system in male crickets.
Article Snippet: The purified DNAs were inserted into pGEM-T vectors (Promega Co.) to construct cDNA-inserted plasmids, which were transformed into Escherichia coli to amplify.

Article Title: Hypermethylation-Mediated lncRNA MAGI2-AS3 Downregulation Facilitates Malignant Progression of Laryngeal Squamous Cell Carcinoma via Interacting With SPT6
Article Snippet: Fifty nanograms of bisulfite-modified DNA was amplified using PCR, and the products were inserted into pGEM-T vectors (Promega, USA), and the above specimens were sequenced (Sangon Biotech).

Article Title: Development of DNA aptamers universally bound to single-chain fragment variables and their applications in bioprocess monitoring.
Article Snippet: Purified PCR products were subcloned into pGEM-T vectors (Promega, Madison, WI, USA), amplified using the illustraTM TempliphiTM DNA Amplification Kit (Cytiva, Tokyo, Japan) according to the manufacturer’s protocol, and subjected to sequence analysis (Eurofin Genomics).

Article Title: First report of cucumber mosaic virus infecting Ocimum basilicum in Korea
Article Snippet: Amplicons were cloned in pGEM-T vectors (Promega, Madison, WI), and subjected to sequencing.

Article Title: Reversible promoter demethylation of PDGFD confers gemcitabine resistance through STAT3 activation and RRM1 upregulation.
Article Snippet: Drug resistance is a major problem in cancer treatment with traditional or targeted therapeutics.. Gemcitabine is approved for several human cancers and the first line treatment for locally advanced or metastatic pancreatic ductal adenocarcinoma (PDAC).. However, gemcitabine resistance frequently occurs and is a major problem in successful treatments of these cancers and the mechanism of gemcitabine resistance remains largely unknown.

Article Title: Methylation Mediated Downregulation of TOB1-AS1 and TOB1 Correlates with Malignant Progression and Poor Prognosis of Esophageal Squamous Cell Carcinoma.
Article Snippet: Background TOB1, a member of the transducer of erbB-2 /B-cell translocation gene family, was detected to be downregulated in ESCC by RNA sequencing.. TOB1-AS1, a head-to-head antisense lncRNA with TOB1, was down-regulated in several cancers.. However, the roles of them in esophageal squamous cell carcinoma (ESCC) remained unclarified.

Construct:

Article Title: Stemness Activity Underlying Whole Brain Regeneration in a Basal Chordate.
Article Snippet: Genes were amplified from P. mytiligera cDNAs using gene-specific primers and cloned into pGEM-t vectors using the manufacturer’s protocol (Promega; CAT #A1360).

Article Title: Engineering redirected NF-κB/OIP5 expression programs to enhance tumor responses to chemotherapy in bladder cancer.
Article Snippet: The PCR products were then subcloned using the pGEM-T vectors (Promega, USA) and transformed into DH5a E. coli cells.

Article Title: Two neuropeptide signaling pathways regulate post-mating refractoriness and reproductive system in male crickets.
Article Snippet: The purified DNAs were inserted into pGEM-T vectors (Promega Co.) to construct cDNA-inserted plasmids, which were transformed into Escherichia coli to amplify.

Article Title: Hypermethylation-Mediated lncRNA MAGI2-AS3 Downregulation Facilitates Malignant Progression of Laryngeal Squamous Cell Carcinoma via Interacting With SPT6
Article Snippet: Fifty nanograms of bisulfite-modified DNA was amplified using PCR, and the products were inserted into pGEM-T vectors (Promega, USA), and the above specimens were sequenced (Sangon Biotech).

Article Title: Development of DNA aptamers universally bound to single-chain fragment variables and their applications in bioprocess monitoring.
Article Snippet: Purified PCR products were subcloned into pGEM-T vectors (Promega, Madison, WI, USA), amplified using the illustraTM TempliphiTM DNA Amplification Kit (Cytiva, Tokyo, Japan) according to the manufacturer’s protocol, and subjected to sequence analysis (Eurofin Genomics).

Article Title: First report of cucumber mosaic virus infecting Ocimum basilicum in Korea
Article Snippet: Amplicons were cloned in pGEM-T vectors (Promega, Madison, WI), and subjected to sequencing.

Article Title: Reversible promoter demethylation of PDGFD confers gemcitabine resistance through STAT3 activation and RRM1 upregulation.
Article Snippet: Drug resistance is a major problem in cancer treatment with traditional or targeted therapeutics.. Gemcitabine is approved for several human cancers and the first line treatment for locally advanced or metastatic pancreatic ductal adenocarcinoma (PDAC).. However, gemcitabine resistance frequently occurs and is a major problem in successful treatments of these cancers and the mechanism of gemcitabine resistance remains largely unknown.

Article Title: Methylation Mediated Downregulation of TOB1-AS1 and TOB1 Correlates with Malignant Progression and Poor Prognosis of Esophageal Squamous Cell Carcinoma.
Article Snippet: Background TOB1, a member of the transducer of erbB-2 /B-cell translocation gene family, was detected to be downregulated in ESCC by RNA sequencing.. TOB1-AS1, a head-to-head antisense lncRNA with TOB1, was down-regulated in several cancers.. However, the roles of them in esophageal squamous cell carcinoma (ESCC) remained unclarified.

Transformation Assay:

Article Title: Stemness Activity Underlying Whole Brain Regeneration in a Basal Chordate.
Article Snippet: Genes were amplified from P. mytiligera cDNAs using gene-specific primers and cloned into pGEM-t vectors using the manufacturer’s protocol (Promega; CAT #A1360).

Article Title: Engineering redirected NF-κB/OIP5 expression programs to enhance tumor responses to chemotherapy in bladder cancer.
Article Snippet: The PCR products were then subcloned using the pGEM-T vectors (Promega, USA) and transformed into DH5a E. coli cells.

Article Title: Two neuropeptide signaling pathways regulate post-mating refractoriness and reproductive system in male crickets.
Article Snippet: The purified DNAs were inserted into pGEM-T vectors (Promega Co.) to construct cDNA-inserted plasmids, which were transformed into Escherichia coli to amplify.

Article Title: Hypermethylation-Mediated lncRNA MAGI2-AS3 Downregulation Facilitates Malignant Progression of Laryngeal Squamous Cell Carcinoma via Interacting With SPT6
Article Snippet: Fifty nanograms of bisulfite-modified DNA was amplified using PCR, and the products were inserted into pGEM-T vectors (Promega, USA), and the above specimens were sequenced (Sangon Biotech).

Article Title: Development of DNA aptamers universally bound to single-chain fragment variables and their applications in bioprocess monitoring.
Article Snippet: Purified PCR products were subcloned into pGEM-T vectors (Promega, Madison, WI, USA), amplified using the illustraTM TempliphiTM DNA Amplification Kit (Cytiva, Tokyo, Japan) according to the manufacturer’s protocol, and subjected to sequence analysis (Eurofin Genomics).

Article Title: First report of cucumber mosaic virus infecting Ocimum basilicum in Korea
Article Snippet: Amplicons were cloned in pGEM-T vectors (Promega, Madison, WI), and subjected to sequencing.

Article Title: Reversible promoter demethylation of PDGFD confers gemcitabine resistance through STAT3 activation and RRM1 upregulation.
Article Snippet: Drug resistance is a major problem in cancer treatment with traditional or targeted therapeutics.. Gemcitabine is approved for several human cancers and the first line treatment for locally advanced or metastatic pancreatic ductal adenocarcinoma (PDAC).. However, gemcitabine resistance frequently occurs and is a major problem in successful treatments of these cancers and the mechanism of gemcitabine resistance remains largely unknown.

Article Title: Methylation Mediated Downregulation of TOB1-AS1 and TOB1 Correlates with Malignant Progression and Poor Prognosis of Esophageal Squamous Cell Carcinoma.
Article Snippet: Background TOB1, a member of the transducer of erbB-2 /B-cell translocation gene family, was detected to be downregulated in ESCC by RNA sequencing.. TOB1-AS1, a head-to-head antisense lncRNA with TOB1, was down-regulated in several cancers.. However, the roles of them in esophageal squamous cell carcinoma (ESCC) remained unclarified.

Polymerase Chain Reaction:

Article Title: Stemness Activity Underlying Whole Brain Regeneration in a Basal Chordate.
Article Snippet: Genes were amplified from P. mytiligera cDNAs using gene-specific primers and cloned into pGEM-t vectors using the manufacturer’s protocol (Promega; CAT #A1360).

Article Title: Engineering redirected NF-κB/OIP5 expression programs to enhance tumor responses to chemotherapy in bladder cancer.
Article Snippet: The PCR products were then subcloned using the pGEM-T vectors (Promega, USA) and transformed into DH5a E. coli cells.

Article Title: Two neuropeptide signaling pathways regulate post-mating refractoriness and reproductive system in male crickets.
Article Snippet: The purified DNAs were inserted into pGEM-T vectors (Promega Co.) to construct cDNA-inserted plasmids, which were transformed into Escherichia coli to amplify.

Article Title: Hypermethylation-Mediated lncRNA MAGI2-AS3 Downregulation Facilitates Malignant Progression of Laryngeal Squamous Cell Carcinoma via Interacting With SPT6
Article Snippet: Fifty nanograms of bisulfite-modified DNA was amplified using PCR, and the products were inserted into pGEM-T vectors (Promega, USA), and the above specimens were sequenced (Sangon Biotech).

Article Title: Development of DNA aptamers universally bound to single-chain fragment variables and their applications in bioprocess monitoring.
Article Snippet: Purified PCR products were subcloned into pGEM-T vectors (Promega, Madison, WI, USA), amplified using the illustraTM TempliphiTM DNA Amplification Kit (Cytiva, Tokyo, Japan) according to the manufacturer’s protocol, and subjected to sequence analysis (Eurofin Genomics).

Article Title: First report of cucumber mosaic virus infecting Ocimum basilicum in Korea
Article Snippet: Amplicons were cloned in pGEM-T vectors (Promega, Madison, WI), and subjected to sequencing.

Article Title: Reversible promoter demethylation of PDGFD confers gemcitabine resistance through STAT3 activation and RRM1 upregulation.
Article Snippet: Drug resistance is a major problem in cancer treatment with traditional or targeted therapeutics.. Gemcitabine is approved for several human cancers and the first line treatment for locally advanced or metastatic pancreatic ductal adenocarcinoma (PDAC).. However, gemcitabine resistance frequently occurs and is a major problem in successful treatments of these cancers and the mechanism of gemcitabine resistance remains largely unknown.

Article Title: Methylation Mediated Downregulation of TOB1-AS1 and TOB1 Correlates with Malignant Progression and Poor Prognosis of Esophageal Squamous Cell Carcinoma.
Article Snippet: Background TOB1, a member of the transducer of erbB-2 /B-cell translocation gene family, was detected to be downregulated in ESCC by RNA sequencing.. TOB1-AS1, a head-to-head antisense lncRNA with TOB1, was down-regulated in several cancers.. However, the roles of them in esophageal squamous cell carcinoma (ESCC) remained unclarified.

Amplification:

Article Title: Stemness Activity Underlying Whole Brain Regeneration in a Basal Chordate.
Article Snippet: Genes were amplified from P. mytiligera cDNAs using gene-specific primers and cloned into pGEM-t vectors using the manufacturer’s protocol (Promega; CAT #A1360).

Article Title: Engineering redirected NF-κB/OIP5 expression programs to enhance tumor responses to chemotherapy in bladder cancer.
Article Snippet: The PCR products were then subcloned using the pGEM-T vectors (Promega, USA) and transformed into DH5a E. coli cells.

Article Title: Two neuropeptide signaling pathways regulate post-mating refractoriness and reproductive system in male crickets.
Article Snippet: The purified DNAs were inserted into pGEM-T vectors (Promega Co.) to construct cDNA-inserted plasmids, which were transformed into Escherichia coli to amplify.

Article Title: Hypermethylation-Mediated lncRNA MAGI2-AS3 Downregulation Facilitates Malignant Progression of Laryngeal Squamous Cell Carcinoma via Interacting With SPT6
Article Snippet: Fifty nanograms of bisulfite-modified DNA was amplified using PCR, and the products were inserted into pGEM-T vectors (Promega, USA), and the above specimens were sequenced (Sangon Biotech).

Article Title: Development of DNA aptamers universally bound to single-chain fragment variables and their applications in bioprocess monitoring.
Article Snippet: Purified PCR products were subcloned into pGEM-T vectors (Promega, Madison, WI, USA), amplified using the illustraTM TempliphiTM DNA Amplification Kit (Cytiva, Tokyo, Japan) according to the manufacturer’s protocol, and subjected to sequence analysis (Eurofin Genomics).

Article Title: First report of cucumber mosaic virus infecting Ocimum basilicum in Korea
Article Snippet: Amplicons were cloned in pGEM-T vectors (Promega, Madison, WI), and subjected to sequencing.

Article Title: Reversible promoter demethylation of PDGFD confers gemcitabine resistance through STAT3 activation and RRM1 upregulation.
Article Snippet: Drug resistance is a major problem in cancer treatment with traditional or targeted therapeutics.. Gemcitabine is approved for several human cancers and the first line treatment for locally advanced or metastatic pancreatic ductal adenocarcinoma (PDAC).. However, gemcitabine resistance frequently occurs and is a major problem in successful treatments of these cancers and the mechanism of gemcitabine resistance remains largely unknown.

Article Title: Methylation Mediated Downregulation of TOB1-AS1 and TOB1 Correlates with Malignant Progression and Poor Prognosis of Esophageal Squamous Cell Carcinoma.
Article Snippet: Background TOB1, a member of the transducer of erbB-2 /B-cell translocation gene family, was detected to be downregulated in ESCC by RNA sequencing.. TOB1-AS1, a head-to-head antisense lncRNA with TOB1, was down-regulated in several cancers.. However, the roles of them in esophageal squamous cell carcinoma (ESCC) remained unclarified.

DNA Amplification:

Article Title: Stemness Activity Underlying Whole Brain Regeneration in a Basal Chordate.
Article Snippet: Genes were amplified from P. mytiligera cDNAs using gene-specific primers and cloned into pGEM-t vectors using the manufacturer’s protocol (Promega; CAT #A1360).

Article Title: Engineering redirected NF-κB/OIP5 expression programs to enhance tumor responses to chemotherapy in bladder cancer.
Article Snippet: The PCR products were then subcloned using the pGEM-T vectors (Promega, USA) and transformed into DH5a E. coli cells.

Article Title: Two neuropeptide signaling pathways regulate post-mating refractoriness and reproductive system in male crickets.
Article Snippet: The purified DNAs were inserted into pGEM-T vectors (Promega Co.) to construct cDNA-inserted plasmids, which were transformed into Escherichia coli to amplify.

Article Title: Hypermethylation-Mediated lncRNA MAGI2-AS3 Downregulation Facilitates Malignant Progression of Laryngeal Squamous Cell Carcinoma via Interacting With SPT6
Article Snippet: Fifty nanograms of bisulfite-modified DNA was amplified using PCR, and the products were inserted into pGEM-T vectors (Promega, USA), and the above specimens were sequenced (Sangon Biotech).

Article Title: Development of DNA aptamers universally bound to single-chain fragment variables and their applications in bioprocess monitoring.
Article Snippet: Purified PCR products were subcloned into pGEM-T vectors (Promega, Madison, WI, USA), amplified using the illustraTM TempliphiTM DNA Amplification Kit (Cytiva, Tokyo, Japan) according to the manufacturer’s protocol, and subjected to sequence analysis (Eurofin Genomics).

Article Title: First report of cucumber mosaic virus infecting Ocimum basilicum in Korea
Article Snippet: Amplicons were cloned in pGEM-T vectors (Promega, Madison, WI), and subjected to sequencing.

Article Title: Reversible promoter demethylation of PDGFD confers gemcitabine resistance through STAT3 activation and RRM1 upregulation.
Article Snippet: Drug resistance is a major problem in cancer treatment with traditional or targeted therapeutics.. Gemcitabine is approved for several human cancers and the first line treatment for locally advanced or metastatic pancreatic ductal adenocarcinoma (PDAC).. However, gemcitabine resistance frequently occurs and is a major problem in successful treatments of these cancers and the mechanism of gemcitabine resistance remains largely unknown.

Article Title: Methylation Mediated Downregulation of TOB1-AS1 and TOB1 Correlates with Malignant Progression and Poor Prognosis of Esophageal Squamous Cell Carcinoma.
Article Snippet: Background TOB1, a member of the transducer of erbB-2 /B-cell translocation gene family, was detected to be downregulated in ESCC by RNA sequencing.. TOB1-AS1, a head-to-head antisense lncRNA with TOB1, was down-regulated in several cancers.. However, the roles of them in esophageal squamous cell carcinoma (ESCC) remained unclarified.

Sequencing:

Article Title: Stemness Activity Underlying Whole Brain Regeneration in a Basal Chordate.
Article Snippet: Genes were amplified from P. mytiligera cDNAs using gene-specific primers and cloned into pGEM-t vectors using the manufacturer’s protocol (Promega; CAT #A1360).

Article Title: Engineering redirected NF-κB/OIP5 expression programs to enhance tumor responses to chemotherapy in bladder cancer.
Article Snippet: The PCR products were then subcloned using the pGEM-T vectors (Promega, USA) and transformed into DH5a E. coli cells.

Article Title: Two neuropeptide signaling pathways regulate post-mating refractoriness and reproductive system in male crickets.
Article Snippet: The purified DNAs were inserted into pGEM-T vectors (Promega Co.) to construct cDNA-inserted plasmids, which were transformed into Escherichia coli to amplify.

Article Title: Hypermethylation-Mediated lncRNA MAGI2-AS3 Downregulation Facilitates Malignant Progression of Laryngeal Squamous Cell Carcinoma via Interacting With SPT6
Article Snippet: Fifty nanograms of bisulfite-modified DNA was amplified using PCR, and the products were inserted into pGEM-T vectors (Promega, USA), and the above specimens were sequenced (Sangon Biotech).

Article Title: Development of DNA aptamers universally bound to single-chain fragment variables and their applications in bioprocess monitoring.
Article Snippet: Purified PCR products were subcloned into pGEM-T vectors (Promega, Madison, WI, USA), amplified using the illustraTM TempliphiTM DNA Amplification Kit (Cytiva, Tokyo, Japan) according to the manufacturer’s protocol, and subjected to sequence analysis (Eurofin Genomics).

Article Title: First report of cucumber mosaic virus infecting Ocimum basilicum in Korea
Article Snippet: Amplicons were cloned in pGEM-T vectors (Promega, Madison, WI), and subjected to sequencing.

Article Title: Reversible promoter demethylation of PDGFD confers gemcitabine resistance through STAT3 activation and RRM1 upregulation.
Article Snippet: Drug resistance is a major problem in cancer treatment with traditional or targeted therapeutics.. Gemcitabine is approved for several human cancers and the first line treatment for locally advanced or metastatic pancreatic ductal adenocarcinoma (PDAC).. However, gemcitabine resistance frequently occurs and is a major problem in successful treatments of these cancers and the mechanism of gemcitabine resistance remains largely unknown.

Article Title: Methylation Mediated Downregulation of TOB1-AS1 and TOB1 Correlates with Malignant Progression and Poor Prognosis of Esophageal Squamous Cell Carcinoma.
Article Snippet: Background TOB1, a member of the transducer of erbB-2 /B-cell translocation gene family, was detected to be downregulated in ESCC by RNA sequencing.. TOB1-AS1, a head-to-head antisense lncRNA with TOB1, was down-regulated in several cancers.. However, the roles of them in esophageal squamous cell carcinoma (ESCC) remained unclarified.

Clone Assay:

Article Title: Stemness Activity Underlying Whole Brain Regeneration in a Basal Chordate.
Article Snippet: Genes were amplified from P. mytiligera cDNAs using gene-specific primers and cloned into pGEM-t vectors using the manufacturer’s protocol (Promega; CAT #A1360).

Article Title: Engineering redirected NF-κB/OIP5 expression programs to enhance tumor responses to chemotherapy in bladder cancer.
Article Snippet: The PCR products were then subcloned using the pGEM-T vectors (Promega, USA) and transformed into DH5a E. coli cells.

Article Title: Two neuropeptide signaling pathways regulate post-mating refractoriness and reproductive system in male crickets.
Article Snippet: The purified DNAs were inserted into pGEM-T vectors (Promega Co.) to construct cDNA-inserted plasmids, which were transformed into Escherichia coli to amplify.

Article Title: Hypermethylation-Mediated lncRNA MAGI2-AS3 Downregulation Facilitates Malignant Progression of Laryngeal Squamous Cell Carcinoma via Interacting With SPT6
Article Snippet: Fifty nanograms of bisulfite-modified DNA was amplified using PCR, and the products were inserted into pGEM-T vectors (Promega, USA), and the above specimens were sequenced (Sangon Biotech).

Article Title: Development of DNA aptamers universally bound to single-chain fragment variables and their applications in bioprocess monitoring.
Article Snippet: Purified PCR products were subcloned into pGEM-T vectors (Promega, Madison, WI, USA), amplified using the illustraTM TempliphiTM DNA Amplification Kit (Cytiva, Tokyo, Japan) according to the manufacturer’s protocol, and subjected to sequence analysis (Eurofin Genomics).

Article Title: First report of cucumber mosaic virus infecting Ocimum basilicum in Korea
Article Snippet: Amplicons were cloned in pGEM-T vectors (Promega, Madison, WI), and subjected to sequencing.

Article Title: Reversible promoter demethylation of PDGFD confers gemcitabine resistance through STAT3 activation and RRM1 upregulation.
Article Snippet: Drug resistance is a major problem in cancer treatment with traditional or targeted therapeutics.. Gemcitabine is approved for several human cancers and the first line treatment for locally advanced or metastatic pancreatic ductal adenocarcinoma (PDAC).. However, gemcitabine resistance frequently occurs and is a major problem in successful treatments of these cancers and the mechanism of gemcitabine resistance remains largely unknown.

Article Title: Methylation Mediated Downregulation of TOB1-AS1 and TOB1 Correlates with Malignant Progression and Poor Prognosis of Esophageal Squamous Cell Carcinoma.
Article Snippet: Background TOB1, a member of the transducer of erbB-2 /B-cell translocation gene family, was detected to be downregulated in ESCC by RNA sequencing.. TOB1-AS1, a head-to-head antisense lncRNA with TOB1, was down-regulated in several cancers.. However, the roles of them in esophageal squamous cell carcinoma (ESCC) remained unclarified.



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